中国医药工业杂志 Chinese Journal of Pharmaceuticals 2004, 35(9) 525
姜 华蔡海波
上海 200237)摘要活细胞的选择性截留是实现细胞高活性
并依据预实验作适当修正所建过程模型对过程操作具有预见性通过简单的数学模型对倾斜式沉降器中细胞沉降的过程进行分析该沉降器对活细胞截留效率高于死细胞并可指导沉降装置的设备放大动物细胞中图分类号选择截留A 文章编号模型如单克隆抗体连续灌注培养过程能连续补充新鲜培养基可维持较好的细胞生长环境优点尤为明显离心分离截留
留等
电过滤截留[6,7]等
倾斜式重力沉降器由于设备简单操作简易
在采用上
流式沉降操作的连续灌注培养生物反应器系统中实
现了高密度培养[8,9]
兆伟
工大学硕士学位论文倾斜式沉降器华东理在一定条件下可在下流式沉降过程中具有更好的细胞截留选择性(王长30cm×°Öüûͼ1ÓÉ»ØÁ÷¿Ú2回流至转瓶在连续灌注培养过程中设置溢流口流量与系统灌注速率相等高1cm膜过滤截5]1 实验原理及装置1.1 倾斜式沉降器细胞分离截留的原理由于上流式倾斜沉降在不同速率颗粒间的相互分离效果欠佳在下流式倾斜沉降器中控制沉降条件沉降速率较高的细胞则从2号口流出(图1)而及介
以更好地根据细胞的不同沉降速率(与细胞颗粒大小
和细胞质量密度有关)进行不同细胞群的分离
以往的下流式细胞沉降截留操作都缺乏理论化的参数估算和分析
立了沉降截留过程的简单数学模型
该过程模型对于操作过程具有预测性2003-09-15作者简介Tel男研究方向2—回流口建在适当操作条件下
中国医药工业杂志 Chinese Journal of Pharmaceuticals 2004, 35(9) 527RRV
1 – 溢流口死细胞密度/ 进液口死细胞密度
Fs
¹ÊÆä¹à×¢Á÷ËÙÊܵ½ÏÞÖƶøÄÑÓÚ·Å´ó±¾ÎÄÌá³öµÄÇãбʽÖØÁ¦³Á½µÆ÷ÏÂÁ÷ʽ²Ù×÷¹ý³ÌÖлº½âÁËÉÏÊöÎÊÌâ
ͨ¹ýÄ£Ð͹ÀËã³Á½µ²ÎÊý·ÖÀëÅųö²¿·ÖËÀϸ°û±¾ÊµÑéÖÐ
Ȕ
ϸ°ûºÍËÀϸ°ûµÄ½ØÁôÂÊ·Ö±ðΪ79.34
ʵÑé½á¹ûÒ²ÑéÖ¤Á˹ý³ÌÄ£ÐÍ参考文献[1]Marino M, Corti A, Ippolito A, et al. Effect of bench-scaleculture conditions on murine IgG heterogeneity[J]. BiotechnolBioeng, 1997, 54(1): 17-25.[2]Kadouri A, Spier RE. Some myths and messages concerningthe batch and continuous culture of animal cells[J]. Cytotech-nology , 1997, 24(2): 89-98.[3]Woodside1 SM, Bowen BD, Piret JM. Mammalian cell re-tention devices for stirred perfusion bioreactors[J]. Cyto-technology, 1998, 28(1-3): 163-175.[4]Voisard D, Meuwly F, Ruffieux PA, et al. Potential of cellretention techniques for large-scale high-density perfusionculture of suspended mammalian cells[J]. Biotechnol Bioeng,2003, 82(7): 571-765.[5]Merten OW. Constructive improvement of the ultrasonic sepa-ration device ADI 1015[J]. Cytotechnology, 2000, 34(1-2):175-179.[6]Docoslis A, Kalogerakis N, Behie LA. Dielectrophoretic forcescan be safely used to retain viable cells in perfusion cultures ofanimal cells[J]. Cytotechnology 1999, 30(1-3): 133-142.[7]Docoslis A, Kalogerakis N, Behie LA, et al. A noveldielectrophoresis-based device for the selective retention ofviable cells in cell culture media[J]. Biotechnol Bioeng 1997,54(3): 239-250.[8]Searles JA, Todd P, Kompala DS. Viable cell recycle with aninclined settler in the perfusion culture of suspended recombi-nant Chinese hamster ovary cells[J]. Biotechnol Prog, 1994,10(2): 198-206.[9]Batt BC, Davis RH, Kompala DS. Inclined sedimentation for
selective retention of viable hybridomas in a continuous sus-
pension bioreactor[J]. Biotechnol Prog, 1990, 6(6): 458-464.
动物细胞沉降分离截留过程的模型研究
作者:
作者单位:
刊名:
英文刊名:
年,卷(期):姜华, 迟占有, 蔡海波, 谭文松华东理工大学生物反应器工程国家重点实验室,上海,200237中国医药工业杂志CHINESE JOURNAL OF PHARMACEUTICALS2004,35(9)
参考文献(9条)
1.Marino M;Corti A;Ippolito A Effect of bench-scale culture conditions on murine IgG heterogeneity[外文期刊] 1997(01)
2.Batt BC;Davis RH;Kompala DS Inclined sedimentation for selective retention of viable hybridomas in a continuoussuspension bioreactor 1990(06)
3.Searles JA;Todd P;Kompala DS Viable cell recycle with an inclined settler in the perfusion culture of suspendedrecombinant Chinese hamster ovary cells 1994(02)
4.Docoslis A;Kalogerakis N;Behie LA A novel dielectrophoresis-based device for the selective retention of viable cellsin cell culture media[外文期刊] 1997(03)
5.Docoslis A;Kalogerakis N;Behie LA Dielectrophoretic forces can be safely used to retain viable cells in perfusioncultures of animal cells[外文期刊] 1999(1-3)
6.Merten OW Constructive improvement of the ultrasonic separation device ADI 1015[外文期刊] 2000(1-2)
7.Voisard D;Meuwly F;Ruffieux PA Potential of cell retention techniques for large-scale high-density perfusion cultureof suspended mammalian cells[外文期刊] 2003(07)
8.Woodside1 SM;Bowen BD;Piret JM Mammalian cell retention devices for stirred perfusion bioreactors[外文期刊] 1998(1-3)
9.Kadouri A;Spier RE Some myths and messages concerning the batch and continuous culture of animal cells[外文期刊]1997(02)
本文链接:http://d.g.wanfangdata.com.cn/Periodical_zgyygy200409007.aspx